Fluoro Phoreproperties

Fluorophores Absorption and Emission Data

The table lists the properties of the most commonly used fluorophore for peptide labeling of your interest in the life sciences. The fluorophores absorbance wavelength from 325nm to 743nm (from blue color to red color), the emission wavelength from 386nm to 770nm.

Fluorophores Emission Data

Fluorophore Dyes Ex (nm) Em (nm)
Hydroxycoumarin 325 386
Dansyl340 578
7-Amino-4-methylcoumarin 351 430
methoxycoumarin 360 410
Alexa fluor 345 442
aminocoumarin 350 445
AMC 345 445
Dabcyl 453 --
Cy2 490 510
FAM 495 517
Alexa fluor 488 494 517
Fluorescein FITC 495 519
Alexa fluor 430 430 545
5-Carboxyfluorescein (5-FAM) 492 518
Alexa fluor 532 530 555
HEX 535 556
5-TAMRA 542 568
Cy3 550570
TRITC 547 572
Alexa fluor 546 556 573
Alexa fluor 555 556 573
Dansyl 340 578
R-phycoerythrin (PE) (489) 565 578
Rhodamine Red-X 560 580
Tamara 565 580
Cy3 (512) 550 570
Cy3.5 581 596
Rox 575 602
Alexa fluor 568 578 603
Texas Red 589 615
Alexa fluor 594 590 617
Alexa fluor 621 639
Alexa fluor 633 650 668
Cy5 (625)650 670
Alexa fluor 660 663 690
Cy5.5 675 694
TruRed 490; 675 695
Alexa fluor 680 679 702
Cy7 743 767